px330 cas9 plasmid Search Results


px330 cas9 plasmid  (Addgene inc)
94
Addgene inc px330 cas9 plasmid
Generation of LAMC2 -EGFP knock-in human pancreatic cancer cells. a Design of LAMC2‐EGFP donor and <t>CRISPR/Cas9</t> sgRNA vectors. Blue circle represents the <t>CRISPR/Cas9</t> <t>protein</t> complex and the yellow box underneath illustrates the guide RNA. b qPCR analysis for LAMC2 gene expression in EGFP + and EGFP − cells. Data are normalized to GAPDH and are presented as fold change in gene expression relative to the EGFP − counterpart. c Sphere formation capacity for EGFP + versus EGFP − cells. d Representative images of gelatin degradation for EGFP + versus EGFP − cells. Nuclei were stained with Hoechst 33342 (blue), green represents actin (Alexa Fluor™ 488 Phalloidin) and red illustrates gelatin (Rodhamine). The white dashed line circles indicates the areas of degradation. e Invasive potential of sh empty versus LAMC2 knockdown cells. f qPCR analysis for EMT and MMP2 and MMP10 gene expression for EGFP + versus EGFP − cells. Data are normalized to GAPDH and are presented as fold change in gene expression relative to the EGFP − cells. g Western blot analysis of VIM and MMP2 in EGFP + and EGFP − cells. Parallel Tubulin immunoblotting was performed. * p < 0.05, ** p < 0.005, *** p < 0.0005. n \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\ge 3.$$\end{document} ≥ 3 . Statistical significance was assessed by Student's t-test
Px330 Cas9 Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/px330+cas9+plasmid/pX330-BsaIx2-DD-Cas9+(Plasmid+%2375380)/pmc09609288-152-0-10
Average 94 stars, based on 1 article reviews
px330 cas9 plasmid - by Bioz Stars, 2026-09
94/100 stars
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cas9 plasmid px330  (Addgene inc)
86
Addgene inc cas9 plasmid px330
Generation of LAMC2 -EGFP knock-in human pancreatic cancer cells. a Design of LAMC2‐EGFP donor and <t>CRISPR/Cas9</t> sgRNA vectors. Blue circle represents the <t>CRISPR/Cas9</t> <t>protein</t> complex and the yellow box underneath illustrates the guide RNA. b qPCR analysis for LAMC2 gene expression in EGFP + and EGFP − cells. Data are normalized to GAPDH and are presented as fold change in gene expression relative to the EGFP − counterpart. c Sphere formation capacity for EGFP + versus EGFP − cells. d Representative images of gelatin degradation for EGFP + versus EGFP − cells. Nuclei were stained with Hoechst 33342 (blue), green represents actin (Alexa Fluor™ 488 Phalloidin) and red illustrates gelatin (Rodhamine). The white dashed line circles indicates the areas of degradation. e Invasive potential of sh empty versus LAMC2 knockdown cells. f qPCR analysis for EMT and MMP2 and MMP10 gene expression for EGFP + versus EGFP − cells. Data are normalized to GAPDH and are presented as fold change in gene expression relative to the EGFP − cells. g Western blot analysis of VIM and MMP2 in EGFP + and EGFP − cells. Parallel Tubulin immunoblotting was performed. * p < 0.05, ** p < 0.005, *** p < 0.0005. n \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\ge 3.$$\end{document} ≥ 3 . Statistical significance was assessed by Student's t-test
Cas9 Plasmid Px330, supplied by Addgene inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/px330+cas9+plasmid/pX330-H11r1-2-DD-Cas9+(Plasmid+%2385978)/pmc06557763-28-1-7
Average 86 stars, based on 1 article reviews
cas9 plasmid px330 - by Bioz Stars, 2026-09
86/100 stars
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crispr/cas9  (Promega)
90
Promega crispr/cas9
Generation of LAMC2 -EGFP knock-in human pancreatic cancer cells. a Design of LAMC2‐EGFP donor and <t>CRISPR/Cas9</t> sgRNA vectors. Blue circle represents the <t>CRISPR/Cas9</t> <t>protein</t> complex and the yellow box underneath illustrates the guide RNA. b qPCR analysis for LAMC2 gene expression in EGFP + and EGFP − cells. Data are normalized to GAPDH and are presented as fold change in gene expression relative to the EGFP − counterpart. c Sphere formation capacity for EGFP + versus EGFP − cells. d Representative images of gelatin degradation for EGFP + versus EGFP − cells. Nuclei were stained with Hoechst 33342 (blue), green represents actin (Alexa Fluor™ 488 Phalloidin) and red illustrates gelatin (Rodhamine). The white dashed line circles indicates the areas of degradation. e Invasive potential of sh empty versus LAMC2 knockdown cells. f qPCR analysis for EMT and MMP2 and MMP10 gene expression for EGFP + versus EGFP − cells. Data are normalized to GAPDH and are presented as fold change in gene expression relative to the EGFP − cells. g Western blot analysis of VIM and MMP2 in EGFP + and EGFP − cells. Parallel Tubulin immunoblotting was performed. * p < 0.05, ** p < 0.005, *** p < 0.0005. n \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\ge 3.$$\end{document} ≥ 3 . Statistical significance was assessed by Student's t-test
Crispr/Cas9, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/px330+cas9+plasmid/px330+puro+cas9+plasmid/med_rxiv__2024__11__27__24317972-106-7-10
Average 90 stars, based on 1 article reviews
crispr/cas9 - by Bioz Stars, 2026-09
90/100 stars
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px330-h11r2-3-dd-cas9 (plasmid #85980)  (Addgene inc)
N/A
Standard format: Plasmid sent in bacteria as agar stab
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Image Search Results


Generation of LAMC2 -EGFP knock-in human pancreatic cancer cells. a Design of LAMC2‐EGFP donor and CRISPR/Cas9 sgRNA vectors. Blue circle represents the CRISPR/Cas9 protein complex and the yellow box underneath illustrates the guide RNA. b qPCR analysis for LAMC2 gene expression in EGFP + and EGFP − cells. Data are normalized to GAPDH and are presented as fold change in gene expression relative to the EGFP − counterpart. c Sphere formation capacity for EGFP + versus EGFP − cells. d Representative images of gelatin degradation for EGFP + versus EGFP − cells. Nuclei were stained with Hoechst 33342 (blue), green represents actin (Alexa Fluor™ 488 Phalloidin) and red illustrates gelatin (Rodhamine). The white dashed line circles indicates the areas of degradation. e Invasive potential of sh empty versus LAMC2 knockdown cells. f qPCR analysis for EMT and MMP2 and MMP10 gene expression for EGFP + versus EGFP − cells. Data are normalized to GAPDH and are presented as fold change in gene expression relative to the EGFP − cells. g Western blot analysis of VIM and MMP2 in EGFP + and EGFP − cells. Parallel Tubulin immunoblotting was performed. * p < 0.05, ** p < 0.005, *** p < 0.0005. n \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\ge 3.$$\end{document} ≥ 3 . Statistical significance was assessed by Student's t-test

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: LAMC2 marks a tumor-initiating cell population with an aggressive signature in pancreatic cancer

doi: 10.1186/s13046-022-02516-w

Figure Lengend Snippet: Generation of LAMC2 -EGFP knock-in human pancreatic cancer cells. a Design of LAMC2‐EGFP donor and CRISPR/Cas9 sgRNA vectors. Blue circle represents the CRISPR/Cas9 protein complex and the yellow box underneath illustrates the guide RNA. b qPCR analysis for LAMC2 gene expression in EGFP + and EGFP − cells. Data are normalized to GAPDH and are presented as fold change in gene expression relative to the EGFP − counterpart. c Sphere formation capacity for EGFP + versus EGFP − cells. d Representative images of gelatin degradation for EGFP + versus EGFP − cells. Nuclei were stained with Hoechst 33342 (blue), green represents actin (Alexa Fluor™ 488 Phalloidin) and red illustrates gelatin (Rodhamine). The white dashed line circles indicates the areas of degradation. e Invasive potential of sh empty versus LAMC2 knockdown cells. f qPCR analysis for EMT and MMP2 and MMP10 gene expression for EGFP + versus EGFP − cells. Data are normalized to GAPDH and are presented as fold change in gene expression relative to the EGFP − cells. g Western blot analysis of VIM and MMP2 in EGFP + and EGFP − cells. Parallel Tubulin immunoblotting was performed. * p < 0.05, ** p < 0.005, *** p < 0.0005. n \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\ge 3.$$\end{document} ≥ 3 . Statistical significance was assessed by Student's t-test

Article Snippet: Px330 Cas9 plasmid from Feng Zhang’s laboratory was obtained from addgene (ref. 42230) and was modified by the introduction of a SV40promoter-IRFP expression cassette downstream of Cas9 by FseI – EcoRI.

Techniques: Knock-In, CRISPR, Expressing, Staining, Western Blot